Molecular and Morphological Characterization of a Taxol-Producing Endophytic Fungus, Gliocladium sp., from Taxus baccata
نویسندگان
چکیده
منابع مشابه
Molecular and Morphological Characterization of a Taxol-Producing Endophytic Fungus, Gliocladium sp., from Taxus baccata
The endophytic fungal populations of different tissues of Taxus baccata grown at high altitudes in West Bengal, India were explored. These isolated fungal populations represented different genera, which were screened for taxol production using immunoassay technique. The culture AAT-TS-4(1) that produced taxol was identified as Gliocladium sp. based on its cultural, morphological characteristics...
متن کاملIdentification of a taxol-producing endophytic fungus EFY-36
Morphological and molecular methods were used to identify the statues of an isolate, EFY-36, a taxolproducing endophytic fungus. Based on the morphology of the fungal colony, the mechanism of spore production and the characteristics of the spores, the isolate is Mucor spp. Furthermore, the analysis of 18S ribosome RNA sequence of the isolate was achieved using PCR technology. The results showed...
متن کاملAgrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21.
An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hygromycin B resistance expression vector, pCAMBIA1304'AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly investigated and optimized. PCR amplification a...
متن کاملMolecular Cloning and Characterization of the Phenylalanine Aminomutase Gene From Taxus baccata L.
Background: Taxol is one of the most important anti-cancer drugs, which is obtained from yew trees (<span style="font-variant: normal; ...
متن کاملIsolation and Identification of an Endophytic Fungus Producing Paclitaxel from Taxus Wallichiana Var Mairei.
The objective of this study was to isolate endophytic fungi producing paclitaxel from yew for the purpose of paclitaxel manufacture. Surface sterilized bark of Taxus wallichiana var. mairei was used as source material and potato dextrose agar culture medium was used in isolation of endophytic fungi. Fungal cultures were extracted with a mixture of chloroform / methanol (1:1, v/v) and the paclit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Mycobiology
سال: 2011
ISSN: 1229-8093,2092-9323
DOI: 10.5941/myco.2011.39.3.151